two-dimensional dige experiments Search Results


dige experiments cell pellets  (GE Healthcare)
96
GE Healthcare dige experiments cell pellets
Metabolic comparison of neural and glioma neurospheres. A, functional analysis of the different proteins identified <t>by</t> <t>two-dimensional</t> <t>DIGE</t> analyses was performed using the Ingenuity Pathways Knowledge Base. B, the LDH and G6PD enzymatic activities were measured in the three glioma spheroid cultures and normalized to the activity measured in NSCs (*, p < 0.05). C, expression of the GLUT-1 transporter was measured by quantitative PCR in three glioma CSCs and normalized to the expression measured in NSCs (*, p < 0.05; **, p < 0.01). D, cell number kinetics was assessed in NSCs or glioma spheroids grown in the presence of the indicated carbohydrate substrates. Cell counting was done every other day and culture medium was renewed on day 4.
Dige Experiments Cell Pellets, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2d-dige experiment  (Verlag GmbH)
90
Verlag GmbH 2d-dige experiment
Metabolic comparison of neural and glioma neurospheres. A, functional analysis of the different proteins identified <t>by</t> <t>two-dimensional</t> <t>DIGE</t> analyses was performed using the Ingenuity Pathways Knowledge Base. B, the LDH and G6PD enzymatic activities were measured in the three glioma spheroid cultures and normalized to the activity measured in NSCs (*, p < 0.05). C, expression of the GLUT-1 transporter was measured by quantitative PCR in three glioma CSCs and normalized to the expression measured in NSCs (*, p < 0.05; **, p < 0.01). D, cell number kinetics was assessed in NSCs or glioma spheroids grown in the presence of the indicated carbohydrate substrates. Cell counting was done every other day and culture medium was renewed on day 4.
2d Dige Experiment, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Metabolic comparison of neural and glioma neurospheres. A, functional analysis of the different proteins identified by two-dimensional DIGE analyses was performed using the Ingenuity Pathways Knowledge Base. B, the LDH and G6PD enzymatic activities were measured in the three glioma spheroid cultures and normalized to the activity measured in NSCs (*, p < 0.05). C, expression of the GLUT-1 transporter was measured by quantitative PCR in three glioma CSCs and normalized to the expression measured in NSCs (*, p < 0.05; **, p < 0.01). D, cell number kinetics was assessed in NSCs or glioma spheroids grown in the presence of the indicated carbohydrate substrates. Cell counting was done every other day and culture medium was renewed on day 4.

Journal: The Journal of Biological Chemistry

Article Title: Comparison of Spheroids Formed by Rat Glioma Stem Cells and Neural Stem Cells Reveals Differences in Glucose Metabolism and Promising Therapeutic Applications *

doi: 10.1074/jbc.M111.320028

Figure Lengend Snippet: Metabolic comparison of neural and glioma neurospheres. A, functional analysis of the different proteins identified by two-dimensional DIGE analyses was performed using the Ingenuity Pathways Knowledge Base. B, the LDH and G6PD enzymatic activities were measured in the three glioma spheroid cultures and normalized to the activity measured in NSCs (*, p < 0.05). C, expression of the GLUT-1 transporter was measured by quantitative PCR in three glioma CSCs and normalized to the expression measured in NSCs (*, p < 0.05; **, p < 0.01). D, cell number kinetics was assessed in NSCs or glioma spheroids grown in the presence of the indicated carbohydrate substrates. Cell counting was done every other day and culture medium was renewed on day 4.

Article Snippet: Two-dimensional DIGE Experiments Cell pellets were resuspended in lysis buffer (7 m urea, 2 m thiourea, 4% CHAPS) and proteins were further precipitated using the Two-dimensional Clean-Up kit (Amersham Biosciences).

Techniques: Functional Assay, Activity Assay, Expressing, Real-time Polymerase Chain Reaction, Cell Counting